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Saturday, October 20, 2018

Serum Sorbitol Dehydrogenase Activity as an Indicator of Chemically Induced Liver Damage in Black Bream (Acanthopagrus butcheri)


Curtin University of Technology, Department of Environmental Biology, Kent Street, Bentley, WA, Australia

The value of serum sorbitol dehydrogenase (s-SDH) activity to diagnose chemically induced liver damage in the fish, black bream (Acanthopagrus butcheri), was assessed by intraperitoneal injection with pentachlorophenol sodium salt. Day 3 post-injection s-SDH activity levels were elevated in fish injected at the highest dosage (30 mg Kg-1) compared to control and other treatment groups. Control fish had s-SDH activity levels within the range of 15 to 88 mU mL-1 serum, while at the highest Na-PCP treatment s-SDH activity was 2.5 to 3.5 times higher on day 3 relative to control fish with levels returning to control levels by the 6th day following injection. An increase in the liver somatic index was not identified until day 6 post-injection and histological damage (hyperplasia) was not detected until the 9th day following injection in the high dosage treatment group. This study confirms that changes in s-SDH activity in black bream due to exposure to hepatotoxicants can be detected before physiological or histopathological changes became evident, and that levels of s-SDH in the serum of black bream in excess of 88 mU mL-1 serum indicate potential liver damage.

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